Fig. 2: PITRM1−/− iPSC-derived neurons show the accumulation of APP and increased levels of Aβ peptides. | Molecular Psychiatry

Fig. 2: PITRM1−/− iPSC-derived neurons show the accumulation of APP and increased levels of Aβ peptides.

From: Loss of function of the mitochondrial peptidase PITRM1 induces proteotoxic stress and Alzheimer’s disease-like pathology in human cerebral organoids

Fig. 2

a Representative western blots of APP in PITRM1+/+ and PITRM1−/− iPSC-derived cortical neurons. b Quantification of APP levels normalized to the loading control (mean + SEM; **p < 0.01, two-tailed t test, n = 5). c Quantification of Aβ species and the Aβ42/Aβ40 ratio in the supernatant of PITRM1+/+ and PITRM1−/− iPSC-derived cortical neurons at DIV 35, as performed by Meso Scale immunoassay (mean + SEM; *p < 0.05, ***p < 0.001, two-tailed t test, n = 5). d Gene expression levels of APP in PITRM1+/+ and PITRM1−/− iPSC-derived cortical neurons (mean + SEM; ***p < 0.001, two-tailed t test, n = 5). e Representative western blot of PITRM1+/+ and PITRM1−/− iPSC-derived neurons of total ubiquitinated protein levels. f Quantification of the protein ubiquitination level in PITRM1+/+ and PITRM1−/− iPSC-derived neurons (mean + SEM; **p < 0.01, two-tailed t test, n = 6).

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