Fig. 3: scRNA-seq of cerebral organoids reveals a cell-type-specific impact of PITRM1. | Molecular Psychiatry

Fig. 3: scRNA-seq of cerebral organoids reveals a cell-type-specific impact of PITRM1.

From: Loss of function of the mitochondrial peptidase PITRM1 induces proteotoxic stress and Alzheimer’s disease-like pathology in human cerebral organoids

Fig. 3

a Generation and characterization of cerebral organoids from PITRM1+/+ and PITRM1−/− iPSCs. Immunostaining for β-TUBIII (green), TBR1 (red), and CTIP2 (red) of 2-month-old cerebral organoids. Cell nuclei were counterstained with DAPI (blue). Scale bar, 100 µm. b UMAP dimensionality reduction plot showing the unsupervised clustering of PITRM1+/+ and PITRM1−/− cerebral organoids. Total n = 15,183 cells. c Pie charts showing the frequency of major cell clusters across the genotypes. Cell clusters were determined by expression of specific markers. d UMAP dimensionality reduction plot showing five distinguished clusters with cell-type identities as determined by the expression of specific markers. e Ingenuity pathway analysis (IPA) of DEG in neurons (cluster 1), progenitor cells (cluster 24), glial cells (cluster 3), astrocytes (cluster 13), and microglia (cluster 22).

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