Fig. 5: Monosynaptic GABAergic inputs to sNAcPV neurons mainly stem from the adBNST.
a Schematic showing sNAc injections of AAV-Ef1α-DIO-TVA-eGFP (AAV2/9) virus and AAV-Ef1α-DIO-RG (AAV2/9) on day 1 and RV-EvnA-DsRed on day 21 in PV-Cre mice to retrogradely trace input neurons (red) to NAc shell (yellow, starter neurons). b RV-mediated transsynaptic retrograde tracing of NAc inputs; fluorescence images of NAc region (coronal diagram) in PV-Cre mice (n = 3 mice); scale bar, 100 μm; inset, enlarged view of the region in the dotted white box, showing starter cells (yellow, expressing both eGFP and DsRed, indicated by white arrowheads; scale bar, 50 μm.) c Typical coronal-section planes with distance (anterior-posterior) from bregma showing dsRed-expressing presynaptic neurons retrogradely labeled by NAc injection of virus in PV-Cre mice; scale bar, 100 μm; HIP hippocampus, BLA basolateral amygdala, CeA central amygdala, PVT paraventricular thalamus, mPFC medial prefrontal cortex, LH lateral hypothalamus, VTA ventral tegmental area, BNST bed nucleus of the stria terminalis. d Overview of inputs to NAc PV neurons (n = 3 mice per region giving a total of 12–39 slices). e Sections were co-stained with antibodies against RFPn (RV) (red) and riboprobes (green) for Gad1/2; scale bar, 50 μm. f Approximately 96.1% of RV-infected neurons were co-labeled for Gad1/2 in the adBNST (n = 12 slices, from 3 mice).
