Fig. 4: Transcriptional analysis of NFκB-GR interplay with qPCR. | Molecular Psychiatry

Fig. 4: Transcriptional analysis of NFκB-GR interplay with qPCR.

From: Decoding the temporal nature of brain GR activity in the NFκB signal transition leading to depressive-like behavior

Fig. 4

a Percentages of correlation coefficient in NFκB-GR transcriptional interplay. b Time-lapse heat map of the correlation coefficients of 32 NFκB-response genes with ANAX1 and IκBα (n = 8/group). Temporal network connectivity of (c) LPS-treated mice (n = 8/group) and (d) RU486/LPS-treated mice [n = 8/group except for 12 h post-LPS (n = 7/group)] with four periods. Periods were grouped according to the behavioral outcomes, and seven NFκB-responsive genes were selected for a clear correlation with ANAX1 and IκBα in the middle phase. The connecting lines are colored according to their correlation direction and specificity, as in the insert. e Experimental procedure and immobilization analysis of FST for AAV-based gene silencing in IL-PFC. f Illustrations of the proposed model. GCs, glucocorticoids, seven NFκB-responsive genes; CXC1, IL3, IL6, IL11, IL12, IL16, and IL18. In correlation analysis, only the significant correlation coefficient (P < 0.05) of GraphPad Prism (GraphPad Prism software v7.04, Inc.) was used. Statistical significance was determined by Student’s t-test (*P < 0.05 vs control for immobility analysis) (color figure online).

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