Fig. 2: RCL1 expression in human brain development.
From: RCL1 copy number variants are associated with a range of neuropsychiatric phenotypes
a Bulk transcriptome analysis of prefrontal cortical regions demonstrates RCL1 transcripts enriched during gestational weeks (WKSG) and decrease postnatally, presented as log2 RPKM (reads per kilobase per million) values. DFC dorsolateral prefrontal cortex, VFC ventrolateral prefrontal cortex, MFC medial frontal cortex, OFC orbital frontal cortex. Transcriptome data from Allen Institute for Brain Science Atlas. b Confocal fluorescence image of a mid-gestation human coronal fetal cortex tissue with RCL1 antibody co-labeling with markers, glial fibrillary acidic protein (GFAP), a neuronal marker (NeuN), and nuclei marker (DAPI). RCL1 is lowly, yet uniformly present across developing cortex layers, with an abundant signal within the cortical plate. Scale bar 100 µm. MZ marginal zone, IMZ intermediate (fiber) zone, oSVZ outer sub-ventricular zone, VZ ventricular zone. RCL1 and cell type specific antibody staining with corresponding confocal fluorescence imaging of (c) 9-month-old human cortex (neonatal) and (d) adult cortex (37-year-old). Neuronal marker (NeuN) and global nuclei marker (DAPI) show RCL1 present in both neurons and non-neuronal cell types. Arrows indicate cells colocalized for NeuN and RCL1. Scale bar, 50 µm.
