Fig. 1: Loss of Fkbp5 in the PVN alters HPA axis physiology. | Molecular Psychiatry

Fig. 1: Loss of Fkbp5 in the PVN alters HPA axis physiology.

From: The co-chaperone Fkbp5 shapes the acute stress response in the paraventricular nucleus of the hypothalamus of male mice

Fig. 1

A Cre-LoxP based generation of the Fkbp5PVN−/− mouse line. B Validation of Fkbp5 mRNA expression in the PVN via in situ hybridization (ISH) and RNAscope (for mRNA quantification see Supplementary Fig. 1A). C Fkbp5PVN-/- mice (n = 16) presented reduced body weight, lowered adrenal weights, and increased thymus weights under non-stressed conditions compared to their WT littermates (n = 15). D Corticosterone levels were significantly reduced following a 15 min restraint stress until at least 60 min after stress onset. E A combined Dex/CRH test showed a significantly pronounced response to a low dose of dexamethasone as well as a dampened response to CRH injection. Data are received from mice between 16 and 20 weeks of age and are presented as mean ± SEM. All data were analyzed with a student’s t-test. *p < 0.05, **p < 0.01, and ***p < 0.001.

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