Fig. 3: The reinstatement of endogenous Fkbp5 in the PVN of global Fkbp5 knock-out animals. | Molecular Psychiatry

Fig. 3: The reinstatement of endogenous Fkbp5 in the PVN of global Fkbp5 knock-out animals.

From: The co-chaperone Fkbp5 shapes the acute stress response in the paraventricular nucleus of the hypothalamus of male mice

Fig. 3

A Experimental procedure. B Validation of successful Fkbp5 rescue by ISH (see Supplementary Fig. 5A for mRNA quantification) and comparable WT Fkbp5 expression. C The reinstatement of Fkbp5 in the PVN resulted in increased adrenal weights and elevated morning corticosterone levels under basal conditions D. Furthermore, Fkbp5 re-instated animals displayed a significantly higher corticosterone response after restraint stress E. For comparison of Fkbp5Frt/Frt and WT see control experiments in Supplementary Fig. 4. Data are presented as mean ± SEM. All data were received from mice between 16 and 20 weeks of age and analyzed a with student’s t-test. *p < 0.05.

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