Fig. 4: Fkbp5 manipulation affects phosphorylation of the glucocorticoid receptor (GR). | Molecular Psychiatry

Fig. 4: Fkbp5 manipulation affects phosphorylation of the glucocorticoid receptor (GR).

From: The co-chaperone Fkbp5 shapes the acute stress response in the paraventricular nucleus of the hypothalamus of male mice

Fig. 4

A Animals lacking Fkbp5 in the PVN showed significantly lower phosphorylation at pGRSer203 and higher levels of (B) pGRSer211 and (C) pGRSer234 compared to the control animals. D Fkbp5PVN−/− mice showed no differences in total GR. E Fkbp5PVN OE animals showed the opposite effect on GR phosphorylation with higher phosphorylation on pGrSer203. F In addition, we observed significantly lower phosphorylation at the GR sites Ser211 and (G) Ser234. H Fkbp5 overexpression had no effect on total GR protein level. Representative blots are shown in (D) and (H). Group size for (A)–(H): 6 vs. 6. Data were received from animals between 16 and 20 weeks of age and are presented as relative fold change to control condition, mean ±  SEM, and were analyzed with a two-way ANOVA. *significant genotype effect, (*p < 0.05, **p < 0.01, ***p < 0.001). +significant genotype × stress interaction (+p < 0.05), #significant stress effect (#p < 0.05, ##p < 0.01).

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