Fig. 5: Single-cell RNA sequencing of cells in the PVN of C57Bl/6 male mice under non-stressed conditions. | Molecular Psychiatry

Fig. 5: Single-cell RNA sequencing of cells in the PVN of C57Bl/6 male mice under non-stressed conditions.

From: The co-chaperone Fkbp5 shapes the acute stress response in the paraventricular nucleus of the hypothalamus of male mice

Fig. 5

A Single-cell sequencing depicted several different cell types. With the majority being neurons (38%), ependymal cells (25%), and astrocytes (14%). Fkbp5+ cells are highlighted. B Diversity of Fkbp5+ cell population. C Neurons could be divided mostly into GABAergic (66%) and glutamatergic (Glut, 11%) cells. Furthermore, the well-known stress markers corticotropin-releasing hormone (Crh, 6%), somatostatin (Sst, 5%), oxytocin (Oxt, 2%), and vasopressin (Avp, 1%) could be detected under basal conditions. D Fkbp5+ cells of selected neuronal subpopulations under basal conditions. E RNAscope validation of Fkbp5 mRNA expression in Crh + neurons. F Quantity of Fkbp5+ cells, as well as Fkbp5 mRNA levels, are significantly increased after stress. Data are were received from animals between 8 and 12 weeks of age and are presented as mean ± SEM. For (E): data were analyzed with student’s t-test. ***p < 0.001.

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