Fig. 6: The Crh-specific overexpression of Fkbp5 in the PVN.

A Crh-specific overexpression of Fkbp5 in the PVN was achieved by bilateral injections of a Cre-dependent Fkbp5 overexpression virus in the PVN of CRH-ires-CRE/Ai9 mice. B Validation of Crh-specific Fkbp5 mRNA overexpression in the PVN by ISH and RNAscope. Arrowheads pointing at viral Fkbp5 expressing Crh⁺ neurons (For mRNA quantification see Supplementary Fig. 9D). C Fkbp5^{CRH OE} mice showed significantly increased adrenal weights (n = 10) and reduced thymus weights (n = 9) under non-stressed conditions compared to the controls (n = 10). D Corticosterone levels were mildly, but not significantly increased in Fkbp5^{CRH OE} at 15, 30, and 60 min post-stress (Fkbp5^{CRH OE} n = 10; Control n = 9). E Fkbp5^{CRH OE} mice displayed a decrease in CRH neuronal mEPSC frequency accompanied by an increased amplitude (Fkbp5^{CRH OE} n_mouse = 4; n_neuron = 29; Control n_mouse = 4; n_neuron = 25), which is reflected in two representative recording traces. Data are presented as mean ± SEM. All data were received from animals between 30 and 34 weeks of age and analyzed with the student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, T = 0.05 < p < 0.1, n.s. not significant.