Fig. 2: GABAergic AgRP neurons modulate anxiety and depression by projection to the dBNST. | Molecular Psychiatry

Fig. 2: GABAergic AgRP neurons modulate anxiety and depression by projection to the dBNST.

From: Reciprocal control of obesity and anxiety–depressive disorder via a GABA and serotonin neural circuit

Fig. 2

a Schematic image showing abrupt inactivation of GABAergic efferent of AgRP neurons followed by facilitating GABA signaling within the dBNST. b, c Expression of tdTomato (red) in AgrpnsCre/+::Gad1lox/lox::Gad2lox/lox::Ai14 mice with vehicle (b, control mice) and NB124 (c, KO mice) injection into the third ventricle. Scale bars in (b) for (b, c), 300 μm. d, e Co-localization of tdTomato and anti-AgRP in ARC after treating AgrpnsCre/+::Gad1lox/lox::Gad2lox/lox::Ai14 mice with NB124. Scale bar in (d) for (d, e), 100 μm. f, g Body weight (f), and daily food intake (g) by the control and KO mice. h Representative activity tracers in OFT by mice as described in (b) and (c) with a chronic infusion of either vehicle or BTZ (1 mg/ml) into the dBNST. l–o Time spent in the center zone (l), distance traveled in the center zone (m), the entries into the center zone (n), and total distance traveled in OFT (o). p Representative activity tracers in EPM by mice as described in (h). q–t Time spent in the open arm (q), distance traveled in the open arm (r), the entries into the open arm (s), and total distance traveled in OFT (t). u, v Immobility time in FST (u) and TST (v) by mice as described in (h). w Representative images of the MBT by mice as described in (h). x Percentage of the number of buried marbles in the MBT. Error bars represent mean ± SEM. n = 7–14; *p < 0.05, Control vs KO; one-way ANOVA and followed by Tukey comparisons test. h Firing activity of zsGreen-labeled dBNST neurons before and after treatment with BTZ (50 µM) in AgrpCre/+::Ai14 mice with an injection of AAV-DIO-WGA-zsGreen into the ARC. i Firing activity of zsGreen-labeled dBNST neurons before and after treatment with BTZ (50 µM) in AgrpnsCre/+::Gad1lox/lox::Gad2lox/lox::Ai14 mice with an injection of NB124 and AAV-DIO-WGA:ZsGreen into the ARC. j Firing frequency of the ZsGreen-labeled dBNST neurons described in (H and I). Error bars represent mean ± SEM. n = 11–20 per group; *p < 0.05, Baseline vs BTZ in control mice; and *p < 0.05, Baseline vs BTZ in KO mice; one-way ANOVA and followed by Tukey comparisons test.

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