Fig. 2: Neurensin-2 induction by stress is reversed by antidepressant treatment.

a Schematic of the Chronic Social Defeat Stress (CSDS). Following 10 days of CSDS, stress-sensitive mice (sen) were identified using the social interaction test (SI). These mice were treated with either vehicle (veh) or fluoxetine (flx) for 21 days. b, c Western blot scans (top) and quantification (bottom) of nuclear SMARCA3 (b) and cytosolic Neurensin-2 (c) from hippocampi of non-stressed control or stress-sensitive mice. b Two-way ANOVA; F (1, 13) = 14.32. Interaction, P = 0.005; stress, F = 167.4, P < 0.0001; treatment, F = 73.92, P < 0.0001. *p = 0.037; ***p = 0.0009. c Two-way ANOVA; F (1, 13) = 0.06, interaction, P = 0.059; stress, F = 61.87, P < 0.0001; treatment, F = 18.65, P = 0.001. *p = 0.042; **p = 0.001. d Schematic of experimental design for restraint stress followed by 14 days of flx or veh. e, f Western blot scans (top) and quantification (bottom) of nuclear SMARCA3 (e) and cytosolic Neurensin-2 (f) from hippocampi of control, or stressed mice. e Two-way ANOVA; interaction, F (1, 13) = 0.0003, P = 0.95; stress, F = 18.74, P = 0.0009; treatment, F = 49.84, P < 0.0009. *p = 0.038, **p = 0.001. f Two-way ANOVA; interaction, F (1, 14) = 5.80, P = 0.03; stress, F = 11.09, P = 0.005; treatment, F = 14.78, P = 0.0018. **p = 0.006 for stress, **p = 0.003 for flx. n = 4–5 mice/group. Molecular weights are indicated in KDa.