Fig. 4: Reactive astrocytes in Grk3−/− mice.
From: GRK3 deficiency elicits brain immune activation and psychosis
A Autoradiography using the [3H]-PBR28 ligand for the 18 kDa translocator-protein (TSPO) in hippocampus revealed increased binding in Grk3−/− mice (n = 8) as compared to Grk3+/+ mice (n = 10; P = 0.0085). Representative image of immunostaining for the microglial marker IBA-1 in hippocampus of (B) Grk3+/+ and (C) Grk3−/− mice. Representative images of GFAP immunostaining in hippocampus of (D) Grk3+/+ (E) and Grk3−/− mice. Quantification of the (F) mean fluorescence intensity in 8 Grk3−/− mice and 7 Grk3+/+ revealed significantly increased intensity in Grk3−/− mice (P = 0.044), although (G) not reaching significant measuring cell density (P = 0.13). Scale bar = 200 μm. All group comparisons were performed using Mann–Whitney U tests. All error bars represent SEM. All tests were two-tailed. *P < 0.05, and **P < 0.01.
