Fig. 2: Characterization of iPSCs and NPCs.

a Representative images of iPSC immunofluorescence staining for one line from each group (CTRL, Li-N and Li-R). Nuclear Oct4 and Nanog pluripotency markers are shown in the merged image. Cell nuclei were stained with DAPI. Scale bars: 100 µm. b Key pluripotency marker alkaline phosphatase (AP) staining positively in iPSC colonies from one line representative of each group used in the study. Scale bars: 100 µm. c Karyotype for one representative line from each group assessed by KaryoStat™ analysis. Somatic and sex chromosomes are displayed together. The y-axis shows the log2 ratios depicting the microarray probe’s signal intensities. A CN value of 2 represents a normal copy number state. Chromosomal gains are represented by a value of 3, while chromosomal losses are represented by a value of 1. Pink, green and yellow colors indicate each individual chromosome probe´s raw signal. The blue line represents the normalized probe signal used to identify copy number aberrations. The same ~7000 kb partial chromosomal loss was detected in 2 patient lines (#5 and #6) on chromosome 6 at position q24.3 (see also Supplementary Fig. 1c). d Real-time PCR analysis of the pluripotency marker genes OCT4, SOX2 and NANOG in CTRL and patient-derived iPSCs compared to H9 embryonic stem cells (H9 ESCs) expression levels. Data is presented as mean ± SD. e Real-time PCR characterization of NPC marker genes NESTIN, PAX6, SOX1, SOX2, MSI1, EMX2, and OTX1. Values are given for mRNA expression levels relative to fibroblasts. Data is presented as mean ± SD. f NPC immunofluorescence staining for Nestin, Sox1 and Sox2. Scale bars: 100 µm. g Percentage of cells staining positive for Nestin, Sox2 and Sox1 by counting 300 cells from the immunofluorescence analysis of each line. No differences were found between groups.