Fig. 5: Mapping the extent of unbound drug transport across the BBB on regional and subregional levels using Kp,uu.

a Brain map showing the imaged brain section (red line) and annotated brain regions. b–d Heat maps of regional Kp,uu,ROI values for risperidone, clozapine, and olanzapine (limits are defined by the minimal and maximal mean Kp,uu,ROI values). e–g Individual scatter dot plots of the regional Kp,uu,ROI values. The Kp,uu,brain value measured on the level of the entire coronal section is shown as a reference point, and a Kp,uu,ROI of unity (indicating predominantly passive BBB transport) is indicated by a red dashed line. Error bars represent the standard deviation of the mean (a semilogarithmic scale is used in the panels). Statistically significant (P < 0.01) differences are highlighted. cc corpus callosum, ec external capsule, aca anterior commissure, CgCx cingulate cortex, MCx motor cortex, SCx somatosensory cortex, InCx insular cortex, Pir piriform cortex, CPu caudate putamen, NAc nucleus accumbens, LS lateral septum.