Fig. 1: Rnd2 expression in the mouse DG. | Molecular Psychiatry

Fig. 1: Rnd2 expression in the mouse DG.

From: The atypical Rho GTPase Rnd2 is critical for dentate granule neuron development and anxiety-like behavior during adult but not neonatal neurogenesis

Fig. 1

(A–C) Distribution of Rnd2 transcripts in the hippocampus at postnatal day 14 (P14) (A) and at adult stage (12-week-old mouse) (B, C). The black rectangle in (B) shows the area enlarged in the inset C. D Analysis by real-time PCR of Rnd2 mRNA expression in microdissected DG (SGZ and GCL) at different ages. Data are presented as fold change compared with the expression level at postnatal day 7 (P7) ± s.e.m (n = 4 mice per time point). P postnatal day, W weeks. E Analysis by real-time PCR of Rnd2 mRNA expression along the septo-temporal axis in the adult (12-week-old) microdissected DG. Data are presented as fold change compared with the expression level at the anteroposterior coordinate −1.34 from the Bregma ± s.e.m (n = 5 mice per time point). F Immunostaining for Rnd2 and doublecortin (DCX) in the DG of a 12-week-old mouse (G) Rnd2 immunostained cells in the SGZ and inner GCL of the adult DG. Nuclei were labeled with TOTO. Scale bars represent 200 µm (A–C), 100 µm (F) and 5 µm (G). See also Supplementary Figs. 1–2.

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