Fig. 2: The absence of Rnd2 reduces the survival of adult-born DGNs. | Molecular Psychiatry

Fig. 2: The absence of Rnd2 reduces the survival of adult-born DGNs.

From: The atypical Rho GTPase Rnd2 is critical for dentate granule neuron development and anxiety-like behavior during adult but not neonatal neurogenesis

Fig. 2

A Images of the DG illustrating the reduction of GFP+ cell number after Rnd2 deletion (GFP/Cre panel) compared to control (GFP panel) at 14 days post injection (dpi). No difference was observed at three dpi. Nuclei were labeled with TOTO. B Survival rate of GFP+ cells at different time points. Mean ± s.e.m.; unpaired two-tailed Student’s t-test; *p < 0.05, **p < 0.01 (n = 3–7 mice). C Immunostaining for GFP and activated caspase-3 (caspase-3a) in the DG, 21 days after GFP/Cre virus injection. TOTO labels nuclei and identifies the GCL. Arrowhead shows a non apoptotic cell and arrows indicate apoptotic cells. D Quantification of the percentage of transduced cells that are caspase-3a+ at 14 and 21 dpi. Mean ± s.e.m.; unpaired one-tailed Student’s t-test; *p < 0.05 (n = 4–7 mice). E The retroviral co-injection strategy allows to analyze in the same mice Rnd2-deleted and control new neurons. The graph shows the survival rate of control (RFP+ only) and Rnd2-knockout (GFP+ only and GFP+/RFP+) new neurons in Rnd2flox/flox mice. Mean ± s.e.m.; paired two-tailed Student’s t-test; **p < 0.01 (n = 7–8 mice). Scale bars represent 50 µm (A) and 10 µm (C). See also Supplementary Figs. 3–6.

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