Fig. 7: Rnd2 suppression in neonatally-born DGNs only affects their dendritic development.

A Distribution of Rnd2 transcripts in the mouse hippocampus at P0. B Analysis of the ratio of total GFP+ to total RFP+ cells in Rnd2^flox/flox mice after injection at P0 of a mix of GFP and RFP expressing retroviruses or GFP/Cre and RFP expressing retroviruses. Mean ± s.e.m, n = 3–6 mice. C Representative images and (D) quantification of the relative position of GFP+ neurons within the GCL, 21 days after GFP or GFP/Cre retrovirus injection at P0. Mean ± s.e.m. (n = 4 mice). E Representative tracings of control and Rnd2-deleted neonatally-born DGNs at 21 dpi. F–H Quantification of the cell body area (F), the total dendritic length (G) and the number of nodes (H) 21 days after P0 injection. Mean ± s.e.m.; paired two-tailed Student’s t-test; *p < 0.05, **p < 0.01 (n = 4 mice, a minimum of three cells were analyzed per animal). I Time spent in the open arms (in % of time spent in the open arms and in the closed arms) and (J) number of total entries in the elevated plus maze. K Time to escape and (L) time spent in the lit area in the light/dark test. M z-anxiety score calculated by averaging z-score values of the different tests used to assess anxiety-like behavior. Mean ± s.e.m., GFP n = 9 and GFP/Cre n = 8 mice. Scale bars represent 100 µm (A) and 20 µm (C, E). See also Supplementary Figs. 12–13.