Fig. 4: SUR1/Kir6.2 regulates NEP expression and activity. | Molecular Psychiatry

Fig. 4: SUR1/Kir6.2 regulates NEP expression and activity.

From: Somatostatin-evoked Aβ catabolism in the brain: Mechanistic involvement of α-endosulfine-KATP channel pathway

Fig. 4

A, B Immunostaining of NEP in hippocampi of WT and ABCC8 KO mice (n = 6 for each group). Scale bar represents 500 µm. C NEP ELISA with hippocampi of WT, heterozygous and homozygous ABCC8 KO mice (n = 5 for each group). D NEP activity in hippocampi of 3-month-old WT and Abcc8 KO mice (n = 5 for each group). E Aβ40 ELISA of hippocampi from 3-month-old WT and Abcc8 KO mice (n = 4 for each group). F Aβ42 ELISA of hippocampi from 3-month-old WT and Abcc8 KO mice (n = 4 for each group). G Aβ40 ELISA of hippocampi from 3-month-old Mme KO and Abcc8/Mme dKO mice (n = 4 for each group). H Aβ42 ELISA of hippocampi from 3-month-old Mme KO and Abcc8/Mme dKO mice (n = 4 for each group). I Immunoblotting of APP, CTFs, IDE and ECE-1 in hippocampi of 3-month-old WT and Abcc8 KO mice. J Values indicated in graphs show band intensities for APP, CTFs, IDE, and ECE-1 normalized to that of β-actin (n = 5 for each group). K, L. Immunostaining of NEP in hippocampi of WT and heterozygous Kcnj8 KO and homozygous Kcnj11 KO mice (n = 4 for each group). Scale bar represents 500 µm. M NEP ELISA with hippocampi from WT and heterozygous Kcnj8 KO and homozygous Kcnj11 KO mice (n = 5 for each group). N NEP activity from hippocampi of 3-month-old WT and heterozygous Kcnj8 KO and homozygous Kcnj11 KO mice (WT: n = 8, Kcnj8 KO: n = 6, Kcnj11 KO: n = 5). In (B), (D), (E), (F), the data represent the mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001 (Student’s t test). In (C), (L), (M), (N), the data represent the mean ± SEM.**P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way ANOVA with Turkey’s multiple comparison test).

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