Fig. 5: Extinction success and Meg3-ex10 knockdown alter chromatin states. | Molecular Psychiatry

Fig. 5: Extinction success and Meg3-ex10 knockdown alter chromatin states.

From: Transcriptome and chromatin alterations in social fear indicate association of MEG3 with successful extinction of fear

Fig. 5

A Septal brain slices from mice subjected to social fear extinction and sacrificed 90 min thereafter were used to visualize Meg-ex10 RNA localization by RNAscope. B Pie plot of the percentage of ATAC-seq peaks found in different regions of the genome in SFC−/Ext+, SFC+/Ext+/suc, and SFC+/Ext+/unsuc as well as SFC+ control and SFC+ Meg3-ex10 knockdown groups. Peaks are summarized into 3′ untranslated region (3′ UTR), 5′ untranslated region (5′ UTR), exons, introns, promoter transcription start sites (TSS), transcription termination sites (TTS), intergenic regions, and non-coding regions. C Correlation of ATAC-seq peaks and CUT&RUN H3K27me3 signals for SFC+/Ext+/suc vs. SFC+/Ext+/unsuc. The y-axis shows log2-fold change and numbers between the boxes represent p-values of Wilcoxon rank-sum test (vs. other). Numbers below the names on the x-axis indicate how many peaks meet the criteria (threshold fold change ≥ 33%, p < 0.05). The right panels show representative peaks around the Auts2 (D) and Dclk3 (E) loci, identified by ATAC-seq. Blue colored areas represent Uber_peaks (Auts2: (1)Uber_peak_135193, (2) Uber_peak_135189; Dclk3: (3) Uber_peak_177423, (4) Uber_peak_177395; for details see Supplementary Data 5), where significant differences between SFC+/Ext+/unsuc vs. SFC+/Ext+/suc and SFC+/Ext+ control vs. SFC+/Ext+ knockdown were detected. The middle and right panels represented fold changes (Fc) of all three replicates per group used for ATAC-seq. SFC+/Ext+/suc were normalized to SFC+/Ext+/unsuc, SFC+/Ext+ knockdown to SFC+/Ext+ control.

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