Fig. 3: Shank1 R882H-KI mice exhibit ASD-like core behaviors.

R882H-KI mice exhibited impaired social behavior in the three-chamber social test in Experiment II (n = 14–16 for each genotype) (A, B). A Social interaction measured. S #1, a stranger mouse placed in one chamber. O, a novel object (inverted wire cup) placed in another chamber. B Social novelty preference measured. S #1, an already-investigated familiar mouse. S #2, a novel unfamiliar mouse. C R882H-KI mice manifest increased repetitive behaviors, as shown by burying of more marbles in the MB test (n = 20–25 for each genotype). D R882H-KI mice showed normal exploratory locomotion in the OF test (n = 16 for each genotype). No anxiety-like behavior was exhibited by R882H-KI mice with face validity, as shown by the center distance ratio in the OF test (E), the time in the light compartment in the LD exploration (F) (n = 16 for each genotype), and the time and entries in open arms in the EPM test (G) (n = 16–17 for each genotype). H No deficits in recognition memory in R882H-KI mice, assessed by NOR (n = 13–14 for each genotype). I Normal performance of spatial learning in the BM (n = 13 for each genotype), measured by the number of errors made before reaching the target hole (genotype, P = 0.1921, trial, P < 0.0001, interaction, P = 0.7528). J No deficits in short-term (Day 5) or long-term (Day 12) spatial memory were observed in R882H-KI mice, as indicated by the number of errors made before reaching the target hole. Paired Student’s t-test for A, B. One-way ANOVA for C–H, J. Repeated-measures ANOVA for I. All data are presented as the mean ± SE. ns, no significance, *P < 0.05, **P < 0.01, ***P < 0.001 for the groups compared with WT.