Fig. 2: Increased endogenous GDNF levels reduce dopamine signalling in the PFC. See also Supplementary Fig. S2. | Molecular Psychiatry

Fig. 2: Increased endogenous GDNF levels reduce dopamine signalling in the PFC. See also Supplementary Fig. S2.

From: Elevated endogenous GDNF induces altered dopamine signalling in mice and correlates with clinical severity in schizophrenia

Fig. 2

A Total dopamine levels in the PFC, measured with HPLC. Box plots show median, upper, and lower quartiles, as well as maximum and minimum values. N = 5–10 mice per group. One-way ANOVA, Tukey’s multiple comparisons test. *p < 0.05. B Stimulated dopamine release in slices from ventral tegmental area (VTA) across four consecutive stimulations, measured with fast-scan cyclic voltammetry. Mean ± SEM. n = 2–6 striatal slices per group, one slice per animal. Two-way repeated measures ANOVA, Tukey’s multiple comparisons test. #p < 0.05; ##p < 0.01 between Gdnf wt/wt;Nestin-Cre and Gdnf wt/cHyper;Nestin-Cre mice. C Similar to (B) but showing dopamine re-uptake. Mean ± SEM. n = 2–6 striatal slices per group, one slice per animal. Two-way repeated measures ANOVA, Tukey’s multiple comparisons test. #p < 0.05 between Gdnf wt/wt;Nestin-Cre and Gdnf wt/cHyper;Nestin-Cre mice. D Quantification of the number of dopamine transporter (DAT)-positive synaptic boutons in the prelimbic area in the PFC. Box plots show median, upper, and lower quartiles, as well as maximum and minimum values. N = 4–6 mice per group (average values from n = 2–3 slices per animal). One-way ANOVA, Tukey’s multiple comparisons test. *p < 0.05. E Representative images of immunohistochemistry for DAT showing dopamine synapses in the prelimbic area in the PFC. Scale bar 50 µm.

Back to article page