Fig. 1: trpγ mutant males express increased courtship drive.

Courtship latency (time to initiate courtship; a, e, i), copulation latency (time to copulate; b, f, j), courtship index (c, g, k) and courtship ethogram (d, h, l) for pairs in which males of different genotypes were tested with wild type (Canton-S) females (a–d), pairs in which females of different genotypes were tested with wild type (Canton-S) males (e–h), and pairs in which both males and females of different (but matched) genotypes were tested (i–l). Genotypes for (a–k) are indicated along X-axis of (c, g, k), respectively; results are color-coded such that gray columns correspond to transheterozygous null trpγ mutant alleles (or CNS specific trpγ knockdown), green columns correspond to relevant control genotypes, yellow columns correspond to genomic rescue of the transheterozygous null trpγ mutant alleles, and orange columns correspond to transheterozygous null trpγ mutant alleles fed hyperforin. For figures (d, h, I) the percentage of males that execute licking and attempted copulation behaviors are indicated in striped and non-striped columns, respectively. Results are shown as means ± s.e.m.; numbers within bars of (c, g, k), corresponds to the number of flies tested. Data were analyzed by a one-way ANOVA, followed by Tukey´s post hoc multiple comparison analyses where “****”: p < 0.0001; “***”: p < 0.001; “**”: p < 0.01; “*”: p < 0.05. Only the most important statistical differences are shown in the figure. See Supplementary Table 2 for exact values for all comparisons.