Fig. 4: Withdrawal induces whole transcriptomic changes in mPFCCRF1+ neurons.
A Schematic of isolation of mPFCCRF1+ neurons using fluorescence activated cell sorting from naïve and withdrawn mice for whole transcriptomic analysis. B Volcano plot of average log fold change plotted against the log of the adjusted p-value for expressed genes. Significantly (p < 0.05) upregulated and downregulated genes in withdrawn mice compared to naive are represented in red and blue, respectively. N = 5–6 male mice. C Top ten most significantly impacted pathways based on the differentially expressed genes (DEGs). Log fold change in the DEGs included in the PI3K-AKT signaling pathway where CSF1 is highlighted in yellow (inset). D Network analysis depicting interactions between DEGs. E Top ten gene ontology (GO) terms overrepresented among DEGs in three domains including: biological processes, molecular function, and cellular components. CSF1 is included in yellow highlighted GO terms.
