Fig. 6: Suppressing expression of oligodendroglial Wif1 rescues synaptic loss and behavioral disorders in DISC1-Δ3 mice. | Molecular Psychiatry

Fig. 6: Suppressing expression of oligodendroglial Wif1 rescues synaptic loss and behavioral disorders in DISC1-Δ3 mice.

From: Pathological oligodendrocyte precursor cells revealed in human schizophrenic brains and trigger schizophrenia-like behaviors and synaptic defects in genetic animal model

Fig. 6

a Staining of synaptic elements in the hippocampus of P12 DISC1-Δ3 mice with Wif1-shRNA encoded retrovirus injection. Scramble shRNA encoded retrovirus serves as control. Scale bar, 5 µm. b Quantification of synaptic elements in a. n = 3 mice from each genotype. c Staining of synaptic elements in the hippocampus of P10 Wif1 conditional KO DISC1-Δ3 Wif1 conditional KO mice. Scale bar, 5 µm. d Quantification of synaptic elements in c. n = 6 mice from each genotype. e–g Representative results of sEPSC or sIPSC recording in P10 Wif1 conditional KO DISC1-Δ3 mice. Quantification of sEPSC frequency and amplitude. n = 18, 19 cells from 3 mice of each genotype. Quantification of sIPSC frequency and amplitude. n = 18, 19 cells from 3 mice of each genotype. h Prepulse inhibition test of P23 Wif1 KO DISC1-Δ3 mice. n = 9, 10 mice of each genotype. i Open field test of P23 Wif1 conditional KO DISC1-Δ3 mice. n = 9, 10 mice of each genotype. Plots show individual data and mean ± SD; n.s. not significant, *p < 0.05, **p < 0.01, ***p < 0.001; two-sided Student’s t-test.

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