Fig. 4: Restoring the vHPC GLAST and GLT-1 reverses HFD-induced hyperactivation in the vHPC→NAc glutamatergic transmission and depression-like behavior. | Molecular Psychiatry

Fig. 4: Restoring the vHPC GLAST and GLT-1 reverses HFD-induced hyperactivation in the vHPC→NAc glutamatergic transmission and depression-like behavior.

From: High-fat diet induces depression-like phenotype via astrocyte-mediated hyperactivation of ventral hippocampal glutamatergic afferents to the nucleus accumbens

Fig. 4

a Experimental timelines of studies determining effects of lentiviral overexpression of vHPC GLAST and GLT-1 on activity of vHPC→NAc glutamatergic transmission and depression-like behaviors in CD and HFD mice. b Efficacy of lentiviral overexpression of vHPC GLAST and GLT-1. Left panels show representative micrographs of Western blots. Right panels shows quantitative results. n = 10 mice in CD-GFP group; n = 9 mice in CD-OE group (OE: overexpression); n = 10 mice in HFD-GFP group; n = 10 mice in HFD-OE group. c Results of SPT. n = 9 cages of mice in CD-GFP group; n = 10 cages of mice in CD-OE group; n = 9 cages of mice in HFD-GFP group; n = 10 cages of mice in HFD-OE group. d Results of FST. n = 19 mice in CD-GFP group; n = 20 mice in CD-OE group; n = 18 mice in HFD-GFP group; n = 20 mice in HFD-OE group. Measurements of c-Fos expression in the FG-labeled NAc-projecting neurons in the vHPC of mice. e Representative fluorescence images of c-Fos-immunoreactive cells (green) and FG-labeled NAc-projecting cells (blue) in the vHPC. Scale bar, 100 µm. f Number of FG-labeled cells. g Percentage of FG-labeled cells also expressing c-Fos. n = 10 mice in CD-GFP group; n = 12 mice in CD-OE group; n = 9 mice in HFD-GFP group; n = 10 mice in HFD-OE group. h Body weight of mice during the experimental period. n = 19 mice in CD-GFP group; n = 20 mice in CD-OE group; n = 18 mice in HFD-GFP group; n = 20 mice in HFD-OE group. Measurements of i fasting blood glucose levels, j fasting plasma insulin levels, and k HOMA-IR index in mice. n = 9 mice in CD-GFP group; n = 11 mice in CD-OE group; n = 8 mice in HFD-GFP group; n = 10 mice in HFD-OE group. Results of IPGTT. l Blood glucose levels during IPGTT in mice. m Analysis of AUC of IPGTT results. n = 9 mice in CD-GFP group; n = 11 mice in CD-OE group; n = 8 mice in HFD-GFP group; n = 10 mice in HFD-OE group. Results of IPITT. n Blood glucose levels during IPITT in mice. o Analysis of AUC of IPITT results. n = 9 mice in CD-GFP group; n = 11 mice in CD-OE group; n = 8 mice in HFD-GFP group; n = 10 mice in HFD-OE group. All data are expressed as mean ± SD. n.s., not significant. See also Supplementary Tables S1 and S2 for details of animal usage and statistical test results.

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