Fig. 5: Riluzole corrects HFD-induced hyperactivation of glutamatergic transmissions and depression-like behaviors. | Molecular Psychiatry

Fig. 5: Riluzole corrects HFD-induced hyperactivation of glutamatergic transmissions and depression-like behaviors.

From: High-fat diet induces depression-like phenotype via astrocyte-mediated hyperactivation of ventral hippocampal glutamatergic afferents to the nucleus accumbens

Fig. 5

a Experimental timelines of studies determining effects of a 3-week systemic (intraperitoneal injection) riluzole (RLZ) treatment on expressions of GLAST and GLT-1 in the vHPC, activity of vHPC→NAc glutamatergic transmission, and depression-like behaviors in CD and HFD mice. b Measurements of expressions of GLAST and GLT-1 in the vHPC of mice. Left panels show the representative micrographs of Western blots. Right panel shows quantitative results. n = 9 mice per group. c Results of SPT. n = 8 cages of mice in CD-Veh group (Veh: vehicle control); n = 8 cages of mice in CD-RLZ group; n = 9 cages of mice in HFD-Veh group; n = 10 cages of mice in HFD-RLZ group. d Results of FST. n = 20 mice in CD-Veh group; n = 19 mice in CD-RLZ group; n = 20 mice in HFD-Veh group; n = 22 mice in HFD-RLZ group. Measurements of c-Fos expression in the FG-labeled vHPC→NAc glutamatergic neurons in mice. e Representative fluorescence images of c-Fos-immunoreactive cells (green) and FG-labeled NAc-projecting cells (blue) in the vHPC. Scale bar, 100 µm. f Number of FG-labeled cells. g Percentage of FG-labeled cells also expressing c-Fos. n = 7 mice in CD-Veh group; n = 7 mice in CD-RLZ group; n = 8 mice in HFD-Veh group; n = 8 mice in HFD-RLZ group. h Experimental timelines of studies determining effects of a 7-day central (intra-vHPC infusion) RLZ treatment on expressions of GLAST and GLT-1 in the vHPC, activity of vHPC→NAc glutamatergic transmission, and depression-like behaviors in CD and HFD mice. i Measurements of expressions of GLAST and GLT-1 in the vHPC of mice. Left panels show the representative micrographs of Western blots. Right panel shows quantitative results. n = 10 mice per group. j Results of SPT. n = 10 cages per group. k Results of FST. n = 20 mice per group. Measurements of c-Fos expression in the FG-labeled vHPC→NAc glutamatergic neurons in mice. l Representative fluorescence images of c-Fos-immunoreactive cells (green) and FG-labeled NAc-projecting cells (blue) in the vHPC. Scale bar, 100 µm. m Number of FG-labeled cells. n Percentage of FG-labeled cells also expressing c-Fos. n = 8 mice in CD-Veh group; n = 9 mice in CD-RLZ group; n = 9 mice in HFD-Veh group; n = 10 mice in HFD-RLZ group. All data are expressed as mean ± SD. n.s., not significant. See also Supplementary Tables S1 and S2 for details of animal usage and statistical test results.

Back to article page