Fig. 2: 5-HT1A autoreceptors in the dorsal and median raphe mediate short- and long-term ethanol intake, respectively.
From: Neural serotonergic circuits for controlling long-term voluntary alcohol consumption in mice
A–D Local infusion of F13714 in the dorsal Raphe nucleus (DRN) dose-dependently reduces ethanol intake following short-term (6 weeks) exposure (A, repeated measure one-way ANOVA, n = 14, F(2.054, 26.70) = 34.43, p < 0.0001, with Bonferroni multiple comparison: ***p < 0.001 and ****p < 0.0001 vs vehicle) but has no effects following long-term (12 weeks exposure, (B) repeated measure one-way ANOVA, n = 14, F(2.847, 37.01) = 1.255, p = 0.3035). Cannula placement was verified by histology (C) and only animals with DRN-targeting cannulae were included (D). Local infusion of F13714 in the median Raphe nucleus (MRN) has no effect on ethanol intake following short-term (6 weeks) exposure (E, repeated measure one-way ANOVA, n = 20, F(2.784, 52.89) = 1.277, p = 0.2915) but dose-dependently- reduces ethanol intake following long-term (12 weeks) exposure (F, repeated measure one-way ANOVA, n = 20, F(2.465, 46.83) = 20.13, p < 0.0001, with Bonferroni multiple comparison: *p = 0.0376, **p = 0.0026 and ****p < 0.0001 vs vehicle). Cannula placement was verified by histology (G) and only animals with MRN-targeting cannulae were included (H).
