Fig. 4: Long-term ethanol exposure alters 5-HT neurons innervation in the DG and is reversed by chronic 5-HT1A agonist treatment.
From: Neural serotonergic circuits for controlling long-term voluntary alcohol consumption in mice
5-HT-immunoreactive axons (red) from the dentate gyrus (DG; A), the CA3 region of the hippocampus (CA3; B) and lateral septum (LS; C) of mice exposed to water (water; left panel), ethanol—treated with vehicle (EtOH + veh, middle panel) or ethanol—chronically treated with the 5-HT1A agonist tandospirone (EtOH + tando; right panel) were labelled and the varicosities reconstructed in 3D. Scale bar: 15 μm. Total volume of reconstructed varicosities of mice exposed to water (water; black), ethanol - treated with vehicle (EtOH + veh, light grey) or ethanol - chronically treated with the 5-HT1A agonist tandospirone (EtOH + tando; dark grey) was quantified in the DG (D), CA3 (E) and LS (F). Mean volume ± SEM (in μm3). One-way ANOVA, n = 6, ****p < 0.0001, NS non-significant, p > 0.9999.
