Fig. 3: Loss of hyperforin-induced excitation in dentate gyrus granule cells of TRPC6 KO mice. | Molecular Psychiatry

Fig. 3: Loss of hyperforin-induced excitation in dentate gyrus granule cells of TRPC6 KO mice.

From: Analysis of hyperforin (St. John’s wort) action at TRPC6 channel leads to the development of a new class of antidepressant drugs

Fig. 3

A–C Whole-cell current-clamp recording of granule cells show effects of hyperforin (3 µM) on evoked APs (A, C) and on membrane potential (B). Dashed lines indicate −70 mV, depolarizing ramp was 0–70 pA for WT granule cell and 0–100 pA for TRPC6 KO cell. The hyperforin-induced biphasic response in wt slices was preserved after blocking fast synaptic transmission with kynurenic acid (KA) and picrotoxin (PTX) (C). D, E Voltage-clamp recordings (held at −70 mV) illustrate loss of hyperforin-induced initial inward current in neurons from TRPC6 KO mice (D). The remaining outward current involves K+ channels, as indicated by the loss of current with CsGlu-filled pipette (E). ***p < 0.001.

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