Fig. 5: Interaction studies of TRPC6 C-terminal peptides with hyperforin and membranes. | Molecular Psychiatry

Fig. 5: Interaction studies of TRPC6 C-terminal peptides with hyperforin and membranes.

From: Analysis of hyperforin (St. John’s wort) action at TRPC6 channel leads to the development of a new class of antidepressant drugs

Fig. 5

CD spectra of wild-type TRPC6 peptides in the absence and presence of hyperforin (A). Laurdan fluorescence measurement to monitor membrane fluidity changes caused by hyperforin (white bars), TRPC6 peptide (black bars), and TRPC6mut peptide (red bars) (B). Amino acid sequences from TRPC6 and TRPC6mut are shown in (C). Differences between the two sequences are underlined. Tryptophan fluorescence using residue W782 as a reporter of TRPC6 (D) and TRPC6mut titrated with hyperforin (E). Fluorescence maxima (vertical black line in D and E) were blotted against hyperforin concentration and normalized against fluorescence maxima without hyperforin (F).

Back to article page