Table 1 Plasmids used for dual luciferase reporter assays in HepG2 cells.

From: Analysis of hyperforin (St. John’s wort) action at TRPC6 channel leads to the development of a new class of antidepressant drugs

Reporter gene assay

Plasmid

Plasmid control

Positive control

PXR (24 h)

pGAL4-(UAS)5-TK-LUC

40 ng/well

10 µM SR12813 /

 

pGAL4-PXR-LBD

40 ng/well

10 µM Rifampicin

 

pcDNA3-Rluc

1 ng/well

 
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