Fig. 3: snRNA-seq using iPSC-derived brain organoids from three male patients with OUD.

A UMAP plot of snRNA-seq data, which included 25787 nuclei. B Heatmap of canonical genes to classify UMAP clusters 0–15 from Fig. 3A. Cluster identifiers are labeled on the y axis and canonical marker genes are labelled on the x axis. Darker shades of red and size of the squares represent greater expression of genes and percentage of cell expressing the gene. C The number of cells in each cluster identified. D The number of differentially expressed genes (DEG) in each cluster in response to oxycodone and buprenorphine. E Pathway analysis was performed using the results derived from the differential gene expression analysis in each cell cluster.