Fig. 6: STAT1 expression was significantly induced by oxycodone in patients with OUD. | Molecular Psychiatry

Fig. 6: STAT1 expression was significantly induced by oxycodone in patients with OUD.

From: Single cell transcriptomics reveals distinct transcriptional responses to oxycodone and buprenorphine by iPSC-derived brain organoids from patients with opioid use disorder

Fig. 6

A A schematic outline of procedures used during the differentiation of iPSC-derived forebrain neurons. B Representative examples of staining for neuronal markers: NeuN and TUJ1. C The basal level of protein expression of STAT1 was determined using the iPSC-derived neurons from male and female OUD patients and unaffected controls (n = 3 each group). STAT1 protein expression was significantly higher in OUD patients as compared to unaffected controls. D Representative Western blot images demonstrate that STAT1 expression went up in response to oxycodone treatment in iPSC-derived neurons from patients with OUD. However, STAT1 protein expression in unaffected controls displayed no difference in response to drug treatment. In parallel, p-STAT1 (Ser727) was upregulated in response to oxycodone treatment in iPSC-derived neurons from patients with OUD. veh vehicle, bup buprenorphine, oxy oxycodone.

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