Fig. 2: Ketamine impairs dendrites and synapse of mature SPNs. | Molecular Psychiatry

Fig. 2: Ketamine impairs dendrites and synapse of mature SPNs.

From: Ketamine impairs growth cone and synaptogenesis in human GABAergic projection neurons via GSK-3β and HDAC6 signaling

Fig. 2

A Comparison of the dendrites of SPNs in the vehicle and ketamine groups after 24 h of exposure. Scale bar, 50 μm. B Comparison of the neurite length (n = 22) and neurite number (n = 30) of SPNs in the vehicle and ketamine groups after 24 h of exposure. C Representative MAP2 and synaptophysin antibody-immunostained dendrites of the vehicle and ketamine-treated SPNs on Day 45. The lower panel is the 3D rendered structure, and it showed synaptophysin+ puncta on the dendrite and spine. Scale bar, 10 μm. D, E Spine density (n = 18) and length (n = 23) and synaptic (computed synapse density from dendrites and spine) parameters (n = 15, 21 and 23) were compared between the SPNs treated with ketamine and the control. Data are from H9 and hiPSC derived SPNs. Data, mean ± s.e.m. One way ANOVA. *P < 0.05.

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