Fig. 6: Ketamine enhanced the direct interaction of GSK-3β with HDAC6. | Molecular Psychiatry

Fig. 6: Ketamine enhanced the direct interaction of GSK-3β with HDAC6.

From: Ketamine impairs growth cone and synaptogenesis in human GABAergic projection neurons via GSK-3β and HDAC6 signaling

Fig. 6

A The immunoblotting results of HDAC6 and pHDAC6 (ser22) antibodies in SPNs cotransfected with GSK-3β knockdown siRNA and the measurement of protein levels (n = 3). B Testing HDAC6 transcription after GSK-3β knockdown siRNA transfection by qRT-PCR (n = 3). C Co-IP of GSK-3β and HDAC6 in the vehicle and ketamine-treated SPNs showed that ketamine treatment enhanced the direct interaction. D Representative high resolution images with z-stacks of GSK-3β and HDAC6 immunostaining taken by SIM microscopy in the vehicle and ketamine-treated SPNs. Scale bar, 4 μm. E Comparison of GSK-3β- and HDAC6-positive values of ROI in the SPNs of the vehicle- and ketamine-treated group (n = 5). Data, mean ± s.e.m. Student’s t test. *P < 0.05.

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