Fig. 2: Aβ42/40 ratio and extracellular Aβ42 deposition are elevated in FAD cultures. | Molecular Psychiatry

Fig. 2: Aβ42/40 ratio and extracellular Aβ42 deposition are elevated in FAD cultures.

From: 3D bioengineered neural tissue generated from patient-derived iPSCs mimics time-dependent phenotypes and transcriptional features of Alzheimer’s disease

Fig. 2

A Table reporting iPS lines used across the study. B Sequencing traces of hFAD-2 Clone B and its isogenic control after reversion of the APP V717I mutation (C-hFAD-2 Clone B). C nSolver Cell Enrichment analysis bar plot. D Aβ42/40 MSD analysis quantification in conditioned media. FAD cultures (hFAD-1, hFAD-2 Clone A, hFAD-2 Clone B) and controls (C-hFAD-2 Clone B, BR24, YZ1) plotted as groups are provided at 2 and 4.5 months. CTR 2 mo: n = 10, FAD 2 mo: n = 7, CTR 4.5 mo: n = 12, FAD 4.5 mo: n = 15. One-way ANOVA, Tukey’s post-hoc test. E Amyloid burden quantification (%). FAD cultures (hFAD-1, hFAD-2 Clone A, hFAD-2 Clone B) and controls (C-hFAD-2 Clone B, BR24, YZ1) plotted as groups are provided at 2 and 4.5 months. n = 6. One-way ANOVA, Tukey’s post-hoc test. F Representative 3D reconstructions of hNeuron culture (4.5 months) derived from C-hFAD-2 Clone B and hFAD-2 Clone B stained for MAP2B (magenta) and Aβ42 (green). DAPI and silk autofluorescent signals are in gray. Zoom-in view: green Aβ42 extracellular depositions (white arrows) interspersed among hNeurons (MAP2B, magenta). Leica Falcon SP8 HC PL APO CS2 20×/0.75 IMM. 3.5× Zoom.

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