Fig. 1: Altered expression of genes encoding splicing factors in the hippocampus of ethanol withdrawn rats after chronic exposure and human subjects with AUD.
From: Conserved role for PCBP1 in altered RNA splicing in the hippocampus after chronic alcohol exposure
a, c Rats were fed control liquid diet (C), ethanol liquid diet (E), or ethanol liquid diet followed by 24 h of withdrawal from ethanol diet (W). The dorsal hippocampus was dissected and RNA isolated and subjected to qPCR (n = 10 males and 6 females per group). b, d RNA was isolated from the hippocampus of control (CTL, n = 17 male and 7 females) human subjects and those with AUD (n = 15 male and 10 female) and subjected to qPCR. a Heat map showing the expression of Pcbp1, Snrpa, Snrpb, Eif4a3, Alyref, Lsm4, Sf3a2, Ptbp1, and Pcbp2 relative to the control group for each sex in male and female rat hippocampus. *p < 0.05, **p < 0.01, and ****p < 0.0001 when comparing withdrawal to control, by Sidak’s test after two-way ANOVA. Full statistics are reported in Supplementary Table 4. b Heat map showing the relative expression of PCBP1, SNRPA, SNRPB, EIF4A3, ALYREF, LSM4, SF3A2, PTBP1, and PCBP2 in human postmortem hippocampus. Two-way ANOVA indicated a significant diagnosis effect for PCBP1 and a significant sex effect for SNRPA, SNRPB and LSM4. *p < 0.05, main effect of diagnosis. c Pcbp1 expression in male and female rat hippocampus, shown as mean ± SEM with individual data points as circles. *p < 0.05 when comparing withdrawal vs. control and vs. ethanol within males, **p < 0.01 when comparing withdrawal vs. control within females. ****p < 0.0001, main effect of sex. d PCBP1 expression in postmortem hippocampus of male and female control and AUD subjects. *p < 0.05 Significant diagnosis effect for PCBP1 expression. Data shown as the mean ± SEM with individual data points as circles.
