Fig. 3: HAPLN2 splicing is altered in postmortem hippocampus of human subjects with Alcohol Use Disorder (AUD).
From: Conserved role for PCBP1 in altered RNA splicing in the hippocampus after chronic alcohol exposure
Human postmortem hippocampus of 24 control (n = 17 males and 7 females) and 25 AUD (n = 16 males and 9 females) was used for qPCR of HAPLN2 junctions. a Diagram of HAPLN2 gene depicting junctions C and C’. Primers were located in exons 3 and 4 and are indicated by blue arrows. b Agarose gel shows two bands that after sequencing were identified as containing junctions C’ (150 bp) and C (250 bp). Relative abundance of junctions C (c) and C’ (d), calculated as percentage of spliced isoform (PSI). Relative expression by qPCR with primers spanning junctions C (e) and C’ (f). Data are presented as the mean ± SEM. *p < 0.05 by Student’s t-test.
