Fig. 4: PCBP1 binding is enriched at HAPLN2 splice sites during withdrawal from chronic ethanol exposure in the hippocampus of male rats and AUD subjects.
From: Conserved role for PCBP1 in altered RNA splicing in the hippocampus after chronic alcohol exposure
Male rats were fed control liquid diet (C, n = 9), or ethanol liquid diet followed by 24 h of withdrawal from ethanol diet (W, n = 11). The dorsal hippocampus was dissected and subjected to RNA immunoprecipitation (RIP) with a PCBP1 antibody. a Rat Hapln2 gene structure and illustration of the RIP method. b Graph of the relative enrichment of PCBP1 association near the C and C’ splice junctions in male rat Hapln2. *p < 0.05 by Student’s t test. c Human HAPLN2 gene structure and illustration of the RIP method. d Graph of the relative enrichment of PCBP1 around C and C’ junctions in human HAPLN2. Postmortem human hippocampus tissue from control (CTL, n = 23, 6 females and 17 males) and AUD (n = 25, 10 females and 15 males) were used for RIP with a PCBP1 antibody. Two samples were detected as outliers in the male AUD group and one in the female AUD group and were excluded. **p < 0.001 and ****p < 0.0001 by Sidak’s test after two-way ANOVA. Data are presented as the mean ± SEM.
