Fig. 2: Loss of spines on apical dendrites of PrL pyramidal cells in layers V-VI. | Molecular Psychiatry

Fig. 2: Loss of spines on apical dendrites of PrL pyramidal cells in layers V-VI.

From: Loss of spines in the prelimbic cortex is detrimental to working memory in mice with early-life adversity

Fig. 2

A, B Representative apical dendrites of pyramidal cells in layers V-VI in a control and an ELA mouse. The boxed segments were magnified in bottom panels to show thin (arrows) and mushroom-type (arrowheads) spines. C, D Basal dendrites of layers V-VI pyramidal cells in a control (A) and an ELA (B) mouse. The boxed segments were magnified in right panels to display thin (arrows) and mushroom-type (arrowheads) spines. E–I Change in average density but not type of spines was observed in ELA mice (n = 9) versus controls (n = 8) (**P < 0.01). Two-way RM ANOVA revealed an ELA-related loss of spines (F1,15 = 5.09, P = 0.0393) and segment-dependent difference (F14,210 = 90.46, P < 0.0001). In the ELA mice, loss of spines (G) including both thin (H) and mushroom-type (I) spines was apparent on apical dendrites that were 160–240 µm from the soma of pyramidal cells (post hoc test, *P < 0.05, **P < 0.01). No difference was observed on basal dendrites (P > 0.05). Scale bars = 25 µm (low magnification in A–D), 6 µm (bottom panels in A, B and right panels in C, D).

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