Fig. 1: Mutations in MAPT are sufficient to drive changes in long non-coding RNA (lncRNA) profiles in iPSC-derived neurons.
From: Long non-coding RNA SNHG8 drives stress granule formation in tauopathies
A Diagram of experimental design. B–D Principal component analysis (PCA) of MAPT IVS10 + 16, p.P301L, and p.R406W carriers and their respective isogenic controls using only lncRNAs. Red dots, CRISPR-corrected isogenic controls. Black dots, MAPT mutation carriers. E–G Volcano plots representing the differential expression of lncRNAs in MAPT IVS10 + 16, p.P301L, and p.R406W carriers compared to their respective isogenic controls. Red dots, differentially expressed genes (FDR < 0.05). Blue dots, differentially expressed genes (p < 0.05). Gray dots, not significant. H Venn diagram showing lncRNA overlap among all three MAPT mutations. I Bar graph representing mean log2 foldchange of common differentially expressed lncRNAs. J Heat map of correlation between differentially expressed lncRNAs and differentially expressed protein coding RNA. Correlation coefficient >0.6. K GO terms from the analysis of highly correlated protein coding RNAs.
