Fig. 3: SNHG8 is downregulated in mouse and human brains and interacts with tau, TIA1, FUS, TARDBP, and DDX3X.
From: Long non-coding RNA SNHG8 drives stress granule formation in tauopathies
A Normalized read counts (TPM) of Snhg8 in WT mice and Tau-P301L mice. B Normalized read counts of SNHG8 in control and FTLD-tau (FTLD-MAPT, MAPT IVS10 + 16, and MAPT p.P301L carrier) brains. *p ≤ 0.05. C Normalized read counts of SNHG8 in control, progressive supranuclear palsy (PSP), and Alzheimer’s disease (AD) brains. ***p ≤ 0.001. D CatRAPID interaction profile of 2N4R tau reveals multiple interaction domains with SNHG8. E RNA pull down to measure the interaction between tau and SNHG8 using WT-Tau-(2N4R) transfected HEK293-T cells. Plot shows relative transcript expression of SNHG8 and GAPDH after pull-down with tau antibodies (Tau5 and Tau7) or IgG, control. Data are representative of 3 independent experiments. Bar graph is represented as mean ± SEM. Student’s paired t test was used to determine significance. *p ≤ 0.05. F–I CatRAPID interaction profile of RNA-binding proteins with SNHG8, the colors in the heatmap indicate the interaction score (ranging from −3 to +3) of the individual amino acid and nucleotide pairs.
