Fig. 3: Amphiregulin is a CREB target gene regulated directly by CRTC3. | Molecular Psychiatry

Fig. 3: Amphiregulin is a CREB target gene regulated directly by CRTC3.

From: Amphiregulin normalizes altered circuit connectivity for social dominance of the CRTC3 knockout mouse

Fig. 3

a Heat map showing fold-change values in gene expression after 2 h of treatment with forskolin (FSK) in astrocytes. b Relative AREG mRNA expression in primary cell cultures. astrocytes (n = 12), neurons (n = 14), microglia (n = 8). c Relative amphiregulin (AREG) mRNA expression in astrocytes from WT and CRTC3 KO mice, WT (n = 10), KO (n = 10). d Relative AREG mRNA expression in astrocytes after FSK treatment, WT (n = 6), KO (n = 6). e A schematic diagram (top). A luciferase assay of AREG promoter activity (bottom), All samples (n = 6). f Chromatin immunoprecipitation (ChiP) assay showing occupancy of CRTC3 over the AREG promoter in astrocytes exposed to FSK as indicated, One-way ANOVA, Unpaired t-test, Two-way ANOVA (Tukey’s multiple test), *p < 0.05, **p < 0.01, ***p < 0.001 Error bars, SEM.

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