Fig. 1: Reduced mPFC AMPK activity regulated anxiety after exposure to social stress, and genetic ablation of AMPK increased anxiety-like behaviors in mice. | Molecular Psychiatry

Fig. 1: Reduced mPFC AMPK activity regulated anxiety after exposure to social stress, and genetic ablation of AMPK increased anxiety-like behaviors in mice.

From: Anxiolytic effect of antidiabetic metformin is mediated by AMPK activation in mPFC inhibitory neurons

Fig. 1

a Behavioral scheme for repeated social defeat (RSD) in C57BL/6J mice. In the EPM, (b) representative traces and (c) the time (left) and entries (middle) spent in open arms and total entries (right) in control and RSD mice. In the LDT, (d) the duration (left) in the light box and the latency (right) of the first time entering the dark box from the light box. e The expression of AMPK and p-AMPK in the mPFC and vHPC of control and RSD mice; n = 6–7 mice. f Western blotting results of AMPK KO mouse brains; n = 3 mice. In the LDT, (g) the total time that adult AMPK Flox and AMPK KO mice spent in the light box (left) and the latency of the first time to enter the light box from the dark box (right). (n = 9–10 mice per group). h The representative traces in the EPM, (i) the time (left), and the entries (middle) spent in the open arms of adult AMPK Flox and AMPK KO mice, the total entries shows no change between AMPK Flox and AMPK KO mice (right), n = 9–10 mice. Ns, not significant, *P < 0.05, **P < 0.01 by unpaired Student’s t test.

Back to article page