Fig. 3: TIMP2 regulates dendritic spine plasticity within the dentate gyrus.
From: Neuronal TIMP2 regulates hippocampus-dependent plasticity and extracellular matrix complexity
A Schematic representation of the workflow for dendritic spine quantification in Lucifer Yellow-filled DG granule cells. B Overall dendritic spine density in DG granule cells iontophoretically labeled with Lucifer Yellow using sections isolated from six-month-old WT and TIMP2 KO mice (N = 6 neurons per mouse from N = 4–5 mice per group). C Quantification of the percentage of spines categorized according to “thin” spine heads or (D) “mushroom” spine heads. E Representative deconvolved confocal image of a dendritic segment from WT and TIMP2 KO Lucifer Yellow-labeled brain sections, and the downstream 3D reconstructions, with dendritic segment shown in pink, thin spines in green, stubby in blue, mushroom in red, and filopodia in yellow. Scale bar, 2 μm. Data are represented as mean ± SEM. Nested t-test for comparisons with neuron and mouse as levels. *P < 0.05; **P < 0.01. Data points represent neurons (left) and mice (right) for each group. LY Lucifer Yellow.
