Fig. 3: Methods for activating endogenous expression of a lncRNA.
From: Techniques for investigating lncRNA transcript functions in neurodevelopment
A carefully engineered combination of zinc finger domains or TALE proteins fused to a transcriptional activation domain can be designed to target the lncRNA promoter region. Alternatively, CRISPR activation (CRISPRa) utilizes a catalytically-dead Cas9 protein (dCas9) fused to a transcriptional activator and a gRNA with complementarity to a region near the transcription start site (TSS) of the lncRNA. The gRNA recruits two additional transcriptional activators, via its aptamers for robust transcriptional activation. Created with BioRender.
