Fig. 2: Attenuation of (R)-ketamine’s effect on immobility time via aIC inhibition. | Molecular Psychiatry

Fig. 2: Attenuation of (R)-ketamine’s effect on immobility time via aIC inhibition.

From: (R)-ketamine restores anterior insular cortex activity and cognitive deficits in social isolation-reared mice

Fig. 2

a Experimental timeline and schematic of chemogenetic manipulation of the aIC in social isolation-reared mice and a schematic and representative image illustrating the selective transduction of hM4Di-mCherry at the injection site (highlighted in red). CNO, clozapine-N-oxide. b Immobility time of hM4Di-mCherry-expressing mice treated with 10 mg/kg (R)-ketamine or 20 mg/kg (S)-ketamine in the FST. Statistical analysis was performed using Levene’s test and one-way ANOVA, followed by Bonferroni’s multiple comparison test. Levene’s test: p = 0.153 (vehicle), p = 0.012 (CNO); one-way ANOVA: Vehicle, F(2, 47) = 6.198, p = 0.0041; CNO, F(2, 43) = 6.044, p = 0.0049. *p < 0.05, **p < 0.01. c Immobility time of hM3Dq-mCherry- or mCherry-expressing mice treated with vehicle or CNO in the FST. The number in parentheses indicates the number of mice used in the experiment. Statistical analysis was performed using Levene’s test and unpaired t test. Levene’s test: p = 0.744 (mCherry), p = 0.828 (hM3Dq). *p < 0.05. Data are presented as the mean ± s.e.m.

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