Fig. 1: CDKL5 is expressed and active in excitatory and inhibitory neurons of 20-day old mice brains.
From: Cell type-specific expression, regulation and compensation of CDKL5 activity in mouse brain
A Using a Cre-Lox recombination system, CDKL5 was selectively knocked out in excitatory neurons (Nex-Cre; green), in inhibitory neurons (Vgat-Cre; orange), in astrocytes (Gfap-Cre; purple), or in every cell type (Cdkl5 full KO; gray) and cortex or striatum were collected at P20. B–E Western blots showing expression of CDKL5, total EB2 and tubulin, and level of EB2 S222 phosphorylation (pS222) in, respectively, Cdkl5 full KO, Nex-Cre cKO, Gfap-Cre cKO and Vgat-Cre cKO mice at P20. F–H Quantification of CDKL5 expression, EB2 pS222 and total EB2 expression in P20 Cdkl5 full KO, Nex-Cre cKO, Gfap-Cre cKO and Vgat-Cre cKO mice. Mann–Whitney test. n = 3 animals per genotype with 2 technical replicates. **p ≤ 0.01. *p ≤ 0.05.
