Fig. 3: EB2 S222 phosphorylation is reduced by synaptic NMDAR-dependent calcium influx.
From: Cell type-specific expression, regulation and compensation of CDKL5 activity in mouse brain
A Quantification of EB2 pS222 levels upon 50 μM NMDA treatment of DIV14 rat primary cortical neurons. Welch’s t test. n = 3 technical replicates. B Quantification of EB2 pS222 levels upon 10 μM glycine and 25 μM glutamate treatment of DIV15 rat primary cortical neurons. Welch’s t test. n = 4 technical replicates. C Quantification of EB2 pS222 levels upon 50 μM NMDA treatment of DIV15 rat primary cortical neurons in normal ACSF and in calcium free ACSF. Dunnett’s multiple comparisons test. n = 4 technical replicates. D Quantification of EB2 pS222 levels upon 10 μM MK801 and 50 μM bicuculline pre-treatment (10 min) and 50 μM NMDA treatment of DIV15 rat primary cortical neurons. Dunnett’s multiple comparisons test. n = 4 technical replicates.
